Fig. 3: Skeletal muscle-associated phenotypes of the mAktDKO mice.

a Grip strength and b exercise duration, of the mAktDKO mice at the age of the indicated weeks (n = 3, 4, 5 or 6 mice per group, as shown by the number of data points on the graph, same as below). c ATP contents of gastrocnemius after exercise for 60 minutes at the age of 8 weeks (n = 5 mice) and after exercise for 15 minutes at the age of 24 weeks (n = 5 or 6 mice). d, e Insulin-dependent glucose uptake in, d EDL and e soleus, analyzed ex vivo using 2-DG at the age of the indicated weeks (n = 5 or 6 mice). f–h Ad libitum plasma glucose (PG) before insulin challenge (f), g relative PG after insulin challenge, and h area under the curve (AUC) of relative PG after insulin challenge, in insulin tolerance test (ITT), after intraperitoneal injection of human regular insulin (0.75 U/kg BW, 1.0 U/kg BW, and 1.5 U/kg BW, at the age of 8, 40, and 100 weeks, respectively) (n = 8 or 10 mice at the age of 8 weeks, n = 5 or 8 mice at the age of 40 weeks, and n = 5 or 10 mice at the age of 100 weeks). Values of the data are expressed as mean ± SEM. *P < 0.05, **P < 0.01. Unpaired 2-tailed t-test was used for assessment, and the exact P values are provided in Supplementary Data 2. Source data are provided as a Source Data file.