Fig. 4: Mechanisms underlying the phenotypes of the mAktDKO mice.

a Gene expression, c DNA copy number, of EDL of the mAktDKO mice at the age of the indicated weeks analyzed by RT-PCR (n = 4, 5, 6, or 7 mice per group, as shown by the number of data points on the graph, same as below). b ATPase staining of EDL at the age of 52 weeks. Scale bars: 50 μm. d, e Electron microscopic image(s) of, horizontal sectioning (d), and vertical sectioning (e), of EDL at the age of 60 weeks. e The percentage of mitophagy-like events were quantified (n = 30 random fields from 3 mice). Scale bars: d 1 μm, and e 500 nm. f An autophagy-related protein in gastrocnemius at the age of 40 weeks 4 h after intraperitoneal administration of leupeptin at the dose of 40 mg/kg of BW in an ad libitum-fed state analyzed by western blotting (n = 3 mice per group). g Macroscopic image of SA-BG staining of EDL at the age of 80 weeks. Scale graduation: 1 mm. Values of the data are expressed as mean ± SEM. *P < 0.05, **P < 0.01. Unpaired 2-tailed t-test (a, c, e) and one-way ANOVA (f) were used for assessment, and the exact P values are provided in Supplementary Data 2. Source data are provided as a Source Data file.