Fig. 8: SHOX/shox prevents chondrocyte senescence in WS models. | Nature Communications

Fig. 8: SHOX/shox prevents chondrocyte senescence in WS models.

From: WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome

Fig. 8

a–f Representative flow cytometry plots of three independent biological experiments for senescent analysis among CTR, shWRN1#, and rescue groups in hESC (a–c) and hMSC (d–f). g–j qRT-PCR measurement of P53 and P16Ink4a in CTR, shWRN1#, and rescue groups in hESC (g–h) and hMSC (i–j) on day 14. N = 3 independent biological experiments. k–m Representative FISH analysis of 3 independent experiments of p53 and p16 in WT, wrn−/− mutant, and rescue groups on 3 dpf, 7 dpf, and 14 dpf. Scale bar = 50 µm. Data are presented as the mean ± S.D. Statistical analysis was performed using two-tailed unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page