Fig. 5: Amounts of complex formation between endogenous preys and bait fragments in AP-MS correlate with their corresponding affinities.
From: Quantitative fragmentomics allow affinity mapping of interactomes
A Correlations between enrichment values and affinities measured using PBM baits (upper panels) or PDZ baits (lower panels). For preys containing several PDZ domains, we assumed additivity of association constants (see Methods). On the left side, example correlations are shown for one PDZ (SYNJ2BP) and one PBM (ATP2B4) prey protein. On the right side, only the Pearson correlation coefficient (PCC) is shown for measured preys. Note that correlations are overall weaker than in the case of PDZ preys (upper panels), with an average PCC of 0.5 instead of 0.8. B Correlations between enrichment values obtained from different cell lysates measured using PBM baits (upper panels) or PDZ baits (lower panels). On the left side, example correlations are shown for one PDZ (SYNJ2BP) and one PBM (ATP2B4) prey protein. On the right side, only the Pearson correlation coefficient (PCC) is shown for measured preys. Note that correlations are overall weaker than in the case of PDZ preys (upper panels). Source data are provided as a Source Data file. See Supplementary Data 2 for more details.
