Fig. 6: Proteomic impact of stable HPV16 E6 expression in HEK293T cells and the contribution of its PBM.

A Proteomic changes measured with label free mass spectometry (n = 3) in HEK293T cells stably expressing HPV16 E6 or HPV16 E6ΔPBM compared to a control cell line “IRES”. Dots indicate the detected 2273 proteins. Black dots indicate proteins whose abundances changed significantly in both E6- and E6ΔPBM-expressing cells as compared to control cells. The dotted line indicates the diagonal. B Proteomic difference between the cell lines expressing E6, or E6ΔPBM. The dotted line indicates the statistical significance threshold (P < 0.05, two-tailed unpaired T-test) and dark dots indicate proteins whose statistical difference is higher than this threshold. C Proteins that show statistically significant differences in concentration between E6- and E6ΔPBM-expressing cell lines often change in opposite direction compared to the control cell line. D Relative amounts of 11 PDZ proteins and p53 in the three cell lines measured by the proteomic survey. Relative XIC intensities are shown compared to the intensities of the control cell line. E Relative amounts of 5 PDZ-proteins, as well as p53 and E6AP, quantified by western blot. GAPDH normalized abundancies were compared to the intensities of the control cell line. In D and E, mean and STD were calculated from three individual measurements indicated by black dots, and statistical significance is indicated by * for P < 0.05 and ** for P < 0.01. Two-tailed unpaired t-tests were used to determine statistical significance. Exact p values are provided in the source data file together with the other source data. See Supplementary Fig 4 for results of western blots and Supplementary Data 3 for full proteomic results.