Fig. 2: High resolution cryo-EM structure of Ab 2-7 scFv and Ab 12 Fab complexed SARS-CoV-2 S D614G. | Nature Communications

Fig. 2: High resolution cryo-EM structure of Ab 2-7 scFv and Ab 12 Fab complexed SARS-CoV-2 S D614G.

From: IgG-like bispecific antibodies with potent and synergistic neutralization against circulating SARS-CoV-2 variants of concern

Fig. 2

A Side and (B) top views of a composite 3D cryo-EM reconstruction of Ab 2-7 scFvs bound to the receptor binding domains of SARS-CoV-2 spike ectodomains at 3.0 Å resolution. Individual spike protein protomers are colored red, blue and green. The heavy chain of Ab 2-7 scFv is colored yellow, the light chain is colored tan. C Locally refined 3D cryo-EM reconstruction of antibody binding interface. D Atomic model of the binding interface between Ab 2-7 scFv and the spike RBD. E Side view of 3D EM reconstruction of Ab 12 Fab (purple) bound to the RBD of SARS-CoV-2 spike ectodomain. Overlay of the Ab 2-7 and Ab 12 structures demonstrates that the two antibodies target spatially discrete, non-overlapping epitopes (insert, with the Ab 12 cryo-EM reconstruction shown in gray).

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