Fig. 1: Schematic diagram of overall strategies for kinetic compartmentalization using the non-natural enzyme reaction.
From: Kinetic compartmentalization by unnatural reaction for itaconate production
2-Methylcitrate dehydratase (PrpD) was evolutionarily engineered to alter the substrate preference and catalytic efficiency towards citrate. The desired PrpD mutant has higher activity towards citrate to secure cis-aconitate, resulting in increased itaconate production. The non-natural enzyme exhibits a kinetically compartmentalized effect without actual spatial separation. Citrate (blue); isocitrate (orange); cis-aconitate (green); itaconate (red).
