Table 2 Kinetic parameters of PrpD, PrpDVTL, PrpDVT, and PrpDVL
From: Kinetic compartmentalization by unnatural reaction for itaconate production
Enzyme | Substrate | kcat (s−1) | Km (mM) | kcat/Km (mM−1 s−1) |
|---|---|---|---|---|
PrpD | 2-methylcitrate | 0.045 ± 0.025 | 0.96 ± 0.85 | 0.075 ± 0.054 |
Citrate | 10,561.95 ± 3,427.82 | 66.39 ± 22.03 | 159.37 ± 2.36 | |
cis-Aconitate | 119.03 ± 13.42 | 0.70 ± 0.10 | 170.61 ± 5.65 | |
PrpDVTL | 2-methylcitrate | 0.024 ± 0.008 | 1.96 ± 0.21 | 0.013 ± 0.005 |
Citrate | 9,586.41 ± 1,193.47 | 35.32 ± 5.17 | 272.17 ± 11.72 | |
cis-Aconitate | 81.83 ± 14.14 | 0.72 ± 0.10 | 113.60 ± 14.61 | |
PrpDVT | 2-methylcitrate | 0.023 ± 0.008 | 1.93 ± 0.21 | 0.012 ± 0.006 |
Citrate | 6,093.22 ± 1,122.92 | 14.82 ± 2.38 | 410.08 ± 22.85 | |
cis-Aconitate | 172.08 ± 19.98 | 1.72 ± 0.25 | 100.52 ± 3.18 | |
PrpDVL | 2-methylcitrate | 0.035 ± 0.009 | 1.72 ± 0.54 | 0.021 ± 0.002 |
Citrate | 7,804.72 ± 693.10 | 15.81 ± 1.89 | 494.97 ± 15.22 | |
cis-Aconitate | 96.34 ± 4.55 | 1.52 ± 0.08 | 63.63 ± 3.33 |
