Fig. 3: GABPB1L containing GABP complexes bind the TERT promoter duplication. | Nature Communications

Fig. 3: GABPB1L containing GABP complexes bind the TERT promoter duplication.

From: Conserved features of TERT promoter duplications reveal an activation mechanism that mimics hotspot mutations in cancer

Fig. 3

a, b Schematic of the GABP heterotetramer TF complex binding to the de novo and native ETS motifs of the TERTp with the G228A mutation or c.−100_−79 duplication, respectively. The figures were created with BioRender. Representative gels (c, e, g) and quantification (d, f, h) of EMSAs comparing binding affinity of GABPA-B1L heterodimers to the TERTp c.−100_−79 duplication, TERTp wildtype (native ETS motif only) and G228A mutant (native and de novo ETS motifs) DNA fragments. Abbreviations: TERTp, TERT promoter. Source data are provided as a Source Data file. Values are mean and SD. N = 3 technical replicates.

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