Fig. 3: Parallel cascades with CnTYR, EfTyrDC, CvTAm, and TfNCS to non-natural BIAs.

The amine moiety was derived from an amino acid (substrate 1) using a CnTYR-EfTyrDC cascade. In a parallel pathway, another amino acid (substrate 2) was converted into the aldehyde moiety by a EfTyrDC-CvTAm cascade. Non-natural BIAs were then synthesised using TfNCS.