Table 2 Use of the parallel cascades to produce BIAs 23–31

From: Enzymatic synthesis of benzylisoquinoline alkaloids using a parallel cascade strategy and tyrosinase variants

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  1. aYields were determined by HPLC analysis against products standards. bFor preparative-scale reactions, products were purified by preparative HPLC or extraction method (Supplementary Information). cees were determined by chiral HPLC. Reaction conditions: Details are specific to each cascade. E.g. (entry 1) - 50 mL reaction mixture A (RMA) - HEPES (50 mM, pH 5.5), 4 (2.5 mM, 1 equiv.), CnTYR and EfTyrDC (10% (v/v) lysates), 8 (4 equiv.), PLP (0.5 equiv.), CuSO4 (40 μM) at 25 °C for 6 h. A 50 mL reaction mixture B (RMB) - HEPES (50 mM, pH 7.5), 9 (7.5 mM, 3 equiv.), EfTyrDC and CvTAm (10% and 5% (v/v) lysates, respectively), 8 (3 equiv.), PLP (1.25 mM), 15 (3 equiv.) at 37 °C for 6 h. RMA + RMB were combined, TfNCS (10% (v/v) lysates) added, and the reaction run at 37 °C for 8 h.
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