Fig. 3: SLFN11 binding to DNA.
a ssDNA and dsDNA binding ability of SLFN11 monitored by electrophoretic mobility shift assay. The experiment was performed in duplicates. One representative replicate is shown. b NanoDSF measurements of SLFN11 in the presence of ssDNA and dsDNA. c Cryo-EM reconstruction of the SLFN11wt dimer bound to ssDNA. Top view of ssDNA bound between the helicase N- and C-lobes of SLFN11 with indicated 5´ to 3´ direction. d Detailed view of the DNA binding region with labelled DNA-interacting residues. Mutated residue K652 is highlighted in bold. e Fluorescence anisotropy assay documenting the effect of K652D mutation on the binding of SLFN11 towards 50 nt ssDNA. The data were fit to a cooperative binding equation. Data are represented as mean values + /− SEM from four independent experiments. Source data for a, b, e are provided as a Source Data file.
