Fig. 4: Male grafts within female hosts exhibit increased cytotoxic T cell infiltration compared to other groups. | Nature Communications

Fig. 4: Male grafts within female hosts exhibit increased cytotoxic T cell infiltration compared to other groups.

From: Effects of biological sex mismatch on neural progenitor cell transplantation for spinal cord injury in mice

Fig. 4

a Representative images of CD68 (top rows; cyan) and Iba1 (bottom rows; red) immunoreactivity in each treatment group. The same tissue sections were co-labeled with CD68 and Iba1, so each top and bottom panel show the same field of view. b Quantification of the density of CD68+ cells within grafts. MM (n = 9 mice); MX (n = 7 mice); MF (n = 10 mice); FF (n = 6 mice); FX (n = 6 mice); FM (n = 10 mice). c Quantification of the density of Iba1 immunoreactivity within grafts. MM (n = 8 mice); MX (n = 7 mice); MF (n = 10 mice); FF (n = 6 mice); FX (n = 6 mice); FM (n = 9 mice). MF vs. FM: P = 0.0230. d Representative images of CD3 (top rows; white) and CD8 (bottom rows; yellow) immunoreactivity in grafts. CD3 and CD8 are both labeled with rat anti-mouse antibodies, so the top and bottom rows represent different tissue sections. e Quantification of CD3+ cell density within grafts. MM (n = 9 mice); MX (n = 8 mice); MF (n = 10 mice); FF (n = 7 mice); FX (n = 8 mice); FM (n = 9 mice). f Quantification of CD8+ cell density within grafts. MM (n = 8 mice); MX (n = 7 mice); MF (n = 9 mice); FF (n = 7 mice); FX (n = 6 mice); FM (n = 8 mice). MM vs. FM: P = 0.0001; MX vs. FM: P < 0.0001; MF vs. FM: P < 0.0001; FF vs. FM: P = 0.0002. g, h Quantification of the % of DAPI+ cells in perivascular regions that are CD3+. MM vs. FM: P = 0.0016; MF vs. FM: P = 0.0199. MM (n = 9 mice); MX (n = 4 mice); MF (n = 9 mice); FF (n = 5 mice); FX (n = 6 mice); FM (n = 9 mice). h Quantification of the % of DAPI+ cells in perivascular regions that are CD8+. MM vs. FM: P = 0.0031; MF vs. FM: P = 0.0006; FF vs. FM: P = 0.0011; MX vs. FM: P = 0.0127. MM (n = 9 mice); MX (n = 4 mice); MF (n = 9 mice); FF (n = 5 mice); FX (n = 6 mice); FM (n = 9 mice). i Percentage of T cells that express CD8. MM (n = 8 mice); MX (n = 7 mice); MF (n = 9 mice); FF (n = 7 mice); FX (n = 6 mice); FM (n = 8 mice). MM vs. FM: P = 0.0268; MX vs. FM: P = 0.0030; MF vs. FM: P = 0.0016; FF vs. FM: P = 0.0056. All data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus FM by two-way ANOVA with Sidak’s multiple comparisons test. Scale bars = 100 μm. Source data are provided as a Source Data file. The experiments in panels a and d were performed twice with similar results.

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