Table 2

From: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma

Antibody

Species

Clone

Supplier

Catalog No.

Lot No.

PLA

WB

Confocal

FACS

anti-pan RAS

mouse

C-4

SCBT

sc-166691

J0120

1:100

1:2000

  

anti-mTOR

mouse

6H9B10

Biolegend

659202

B241067

1:50

   

anti-LAMP1

mouse

H4A3

SCBT

sc20011

D1612

1:50

 

1:100

 

anti-CD98 (SLC3A2)

rabbit

 

BETHYL

A304-331A

 

1:500

   

anti-mTOR

rabbit

7C10

CST

2983

16, 19

1:200

   

anti-p-4E-BP1 (S65)

rabbit

 

CST

4165

16

 

1:2000

  

anti-p-p70S6K (T389)

rabbit

108D2

CST

9234

12

 

1:1000

  

anti-4E-BP1

rabbit

53H11

CST

9644

12

 

1:2000

  

anti-p70S6K

rabbit

 

CST

9202

20

 

1:2000

  

anti-MEK1/2

rabbit

D1A5

CST

8727

5

 

1:2000

  

anti-p-MEK1/2 (S217/221)

rabbit

41G9

CST

9154

18

 

1:2000

  

anti-p-PKCα/β (T638/641)

rabbit

 

CST

9375

4

 

1:2000

  

anti-PKCa

rabbit

D7E6E

CST

59754

1

 

1:2000

  

anti-CD98 (SLC3A2)

rabbit

D3F9D

CST

47213

1

 

1:5000

  

anti-GAPDH

mouse

O411

SCBT

sc-47724

E2219

 

1:10,000

  

anti-β-actin

goat

C-11

SCBT

sc-1615

E2314

 

1:10,000

  

anti-CD138 Alexa 647

mouse

MI15

Biolegend

356524

B271942

  

1:40

 

anti-active Caspase 3 APC

rabbit

 

BD

51-68655X

8024887

   

1:100

anti-CD54 APC

mouse

HCD54

Biolegend

322712

B263252

   

1:1000

anti-CD98 (SLC3A2)

mouse

4F2

SCBT

sc-59145

E2314

  

1:200

1:500

anti-BioID2

mouse

 

Novus

nbp2-59941

CRT/17/86

 

1:2000

1:1000

 

anti-mouse Alexa 647

goat

 

CST

4410

10

  

1:1000

 

anti-rabbit Alexa 488

goat

 

CST

4412

18

  

1:1000

 

anti-KRAS

mouse

F234

SCBT

sc-30

B0422

1:100

1:250

1:100

 

anti-NRAS

mouse

F155

SCBT

sc-31

B1517

 

1:500

  

anti-p-RPS6 (S240/244) Pac. Blue

rabbit

D68F8

CST

5018 S

1

   

1:500

anti-RPS6 Alexa 488

rabbit

54D2

CST

5317 S

9

   

1:500

anti-BIM

rabbit

C34C5

CST

2933 S

13

 

1:2000

  

anti-PARP

rabbit

 

CST

9542

3

 

1:4000

  
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