Fig. 3: Identification of the His₁₀-SUMO2 targets upon arsenic-induced PML NB reorganization in APL mice.

a Experimental setup for differential SUMO-proteome analyses upon arsenic, based on large-scale PD of His₁₀-S2 conjugates from three mouse cohorts, each containing 6 Ctrl and 6 His₁₀-S2 APL mice treated (n = 3) or not with arsenic (n = 3), for the indicated time, followed by LC-MS/MS analyses. b Volcano plots showing proteins differentially sumoylated in arsenic- vs untreated His₁₀-S2 APL mice for each cohort; label-free quantification (LFQ) fold changes; p-values, two-sided t-test; dashed line represents p-value threshold (0.05). Targets plotted when significantly enriched in His₁₀-S2 vs Ctrl APL mice (Supplementary Data 1), numbers of identified proteins in each group are indicated (left bars). c Venn diagram showing proteins common to arsenic-modulated His₁₀-S2 targets identified in (b) and previously reported PML partners in ref. 33 and Biogrids; p-value (proportion of PML interactors in arsenic-modulated His₁₀-S2 targets), two-sided standard t-test. d Gene Ontology enrichment analysis (GOBP, gene ontology biological process) of 1h-arsenic-modulated targets, highlighting repressive transcriptional regulation. e Schematic overview of the arsenic-modulated SUMO2 targets (pink) and other members related to PML in the KAP1 complex. f Volcano plot showing His₁₀-SUMO-2 conjugated proteins (blue) identified by LC-MS/MC analysis in His₁₀-S2 Pml^−/− vs Pml^+/+ mESCs (see supplementary Fig 3c for Western blot controls). n = 4 biological replicates; fold changes (log2), p-value (log10), two-sided t-test. g Venn diagram showing proteins common to PML-dependent His₁₀-S2 targets identified in (f) and reported as PML partners as in (c), p-value two-sided t-test.