Fig. 5: PML is required for 2 CL transition and DPPA2 sumoylation in mESCs.

a GSEA gene-set term enrichment analysis of the transcripts deregulated in Pml^−/− vs Pml^+/+ mESCs from microarray analysis; 2C ∩ 2CL geneset: transcripts common to 2C embryo and 2CLcells from (ref. 49). b Transcripts de-regulated in Pml^−/− vs Pml^+/+ mESCs, related to upregulated genes in 2C embryo vs oocytes⁴⁹. Transcripts with significant changes (gray) and key 2CL genes (red) are shown from microarray analysis (Supplementary Data 6). c Mean fold increase of the indicated 2CL transcripts and MaeI as a direct target of DPPA2 (qRT-PCR) n = 6 independent biological replicates, normalized on gapdh and relative to values in Pml^+/+ mESCs, +/−SD, **p < 0.01, two-tailed unpaired Mann–Whitney test. Representative of the three CrispR/Cas9 Pml^−/− clones. d GSEA analyses of RNAseq from Pml^−/− vs Pml^+/+ mESCs unraveling Myc targets and Oxidative phosphorylation as the top two altered pathways (Supplementary Data 6). e PML-dependent arsenic-induced S2-conjugation of DPPA2, PD from the indicated mESCs. Inputs in Supplementary Fig. 4h. Representative data, n = 5 independent biological replicates. f Representative image (left) and percentages of Zscan4+ cells (right), increased among Pml^−/− mESCs, mean ± SD, n = 4 independent experiments; total of 400 (left) and 3000 (right) nuclei from n = 4, *p < 0.05, unpaired two-tailed Student’s t-test. g Mean fold change of the indicated 2CL transcripts, DPPA2 target and pluripotent genes from the indicated mESCs exposed or not to 2i medium. n = 3 independent biological replicates, normalized on gapdh and relative to unpaired values in Pml^+/+ mESCs, +/−SD, **p < 0.01, two-tailed unpaired Mann–Whitney t-test. h FACS analysis to quantify Annexin V staining in Pml^−/− vs Pml^+/+ mESCs exposed or not to 2i medium. Representative n = 3. Source data are provided as a Source Data file.