Fig. 1: Cellular composition of the human thymus and TETs.
From: The immune landscape of human thymic epithelial tumors
(a) Schematic diagram of the overall study design and workflow. (b) t-SNE plots of total cells from human TETs (n = 22) and the normal thymus (n = 3) by CyTOF using a Phenograph clustering scheme. Samples were grouped according to WHO histological subtype, Masaoka stage or our alternative classification, and each color represents an independent group or stage (N, normal human thymus; T1, type 1; T2, type 2; T3, type 3; the abbreviations below are consistent with these definitions). (c) t-SNE plot of total cells from all samples (n = 25) by CyTOF using a Phenograph clustering scheme, colored by cell cluster. (d) Bar plots showing the frequencies of cluster 1 and cluster 2 subsets among the four groups of samples (n = 3, 9, 7 and 6 for N, T1, T2 and T3, respectively. Data are presented as the mean ± s.e.m. P values in the figure were determined by an unpaired two-tailed Student’s t test). (e-f) Sheet and pie charts summarizing samples in each alternative classification by WHO histological subtype (e) or Masaoka stage (f). Each color represents a WHO histological subtype or Masaoka stage. (g) Same t-SNE plot as (c), colored by cell type. (h) t-SNE plot of immune cells (EPCAM-CD45 + ) from all samples obtained by CyTOF using a Phenograph clustering scheme, colored by cell type. (i) t-SNE analysis of immune cells from all samples colored by the relative expression of CyTOF markers (CD45, CD3, CD4, CD8a, CD20, CD14, CD141, CD56, CD11c, CD66b, HLA-DR and CD39). (j) Same t-SNE plots of immune cells as (h) from samples of each group (N, T1, T2, T3), colored by cell type. (k) Bar plots showing the frequencies of the main immune cell subsets among the four groups of samples (n = 3, 9, 7 and 6 for N, T1, T2 and T3, respectively. Data are presented as the mean ± s.e.m. P values in the figure were determined by an unpaired two-tailed Student’s t test). (l) Representative flow cytometric plot of CD3+ T cells and CD3-CD4+CD8+ lymphocytes from TETs of each type. Source data are provided as a Source Data file.
