Fig. 4: Efb stabilizes TRAF3. | Nature Communications

Fig. 4: Efb stabilizes TRAF3.

From: Extracellular fibrinogen-binding protein released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3

Fig. 4

a Immunoblots of WCL from MH-S infected with Newman and ΔEfb (MOI = 25). b Immunoblots of WCL and IP products of WCL from HEK293T cells transfected with indicated plasmids. Ub, wild-type ubiquitin; K48, Ub with a single 48 lysine residue left; K63 Ub with a single 63 lysine residue left. c Immunoblots of IP products of WCL from MH-S infected with Newman and ΔEfb (MOI = 25). d Immunoblots of WCL and IP products from HEK293T cells transfected with indicated plasmids. e Immunoblots of IP products from MH-S infected with Newman and ΔEfb (MOI = 25). f Efb inhibits K48-linked ubiquitin chains on TRAF3 in vitro. g Immunoblots of WCL and IP products from HEK293T cells transfected with indicated plasmids. K/R, replace all lysine of TRAF3 with arginine; R106K, replace 106 arginine of K/R with lysine; R155K, replace 155 arginine of K/R with lysine. R106/155K, replace 106 and 155 arginine of K/R with lysine. Data are representative of three experiments with at least three independent biological replicates. Source data are provided as a Source Data file.

Back to article page