Fig. 3: Optimised CB2 biosensor plate reader assay.
From: Screening microbially produced Δ9-tetrahydrocannabinol using a yeast biosensor workflow
a Optimised workflow for CB2 biosensor strain preparation, sample incubation, and plate reader measurement of 96 samples. Throughput can be linearly scaled with starting culture volume. b THC dose-response curve using the optimised CB2 biosensor plate reader assay over the linear range of GFP output (0.01–10 µM of THC). Data was normalised to the uninduced CB2 biosensor response (1). Experimental measurements are GFP levels per cell as determined on a plate reader and shown as the individual values from three biological replicates. A straight-line curve was fitted using GraphPad Prism linear regression fit, R2 = 0.98.
