Fig. 1: Ensemble of models predicted by AlphaFold-Multimer for the tetrameric augmin subcomplexes TIII and TII.

a Domain representation of the HAUS proteins with schematic representation of the augmin holocomplex. TIII and TII tetramers are indicated together with the corresponding HAUS proteins. Disordered regions are annotated. b Superposition of predicted models for the augmin TIII subcomplex. The ensemble of models was coloured in rainbow scheme from high (blue) to low (red) prediction score. Colour scheme is given. c Left: highest-scoring model coloured according to RMSD against the model with maximum deviation from 0 Å (blue) to 10 Å (red). Colour scheme is given. Right: zoomed views of model segments predicted to be flexible, with the two most extreme conformations shown. d Predicted models for the augmin TII subcomplex superposed according to either the N-terminal (left) or C-terminal half (right) of the subcomplex. The ensemble of models was coloured in rainbow scheme from high (blue) to low (red) prediction score. Same colour scheme as in b. Disordered regions of HAUS6 (399-978) and HAUS8 (1-154) are not shown. e Superposition of TII models with most extreme conformations after alignment according to the C-terminal half of the subcomplex. The apparent hinge between the two halves is indicated (axis with arrows).