Fig. 5: Subunit architecture and conformational plasticity of the augmin holocomplex.
From: The augmin complex architecture reveals structural insights into microtubule branching
a Superposition of predicted models for the augmin TIII H3/H5 arm in complex with TII. The ensemble of models was coloured in rainbow scheme from high (blue) to low (red) prediction score. Colour scheme is given. b Two model segments were fitted into the negative stain EM 3D reconstruction of the augmin holocomplex as rigid bodies: TIII + TII C-clamp (yellow); TII N-clamp (red). c Subunit architecture of the complete augmin holocomplex in the open conformation. Structural features are indicated. Colour scheme is given. d The augmin holocomplex structures in open (grey; from panel c) and closed conformations (purple; from Supplementary Fig. 9), superposed according to TIII. Only TII and the TIII H3/H5-arm are shown. The two conformations are related by opening/closing of the TII clamp by 23°.
