Fig. 6: Structure-guided hypothetical model for augmin recruitment to the microtubule.
From: The augmin complex architecture reveals structural insights into microtubule branching
Left, the augmin holocomplex is recruited to the pre-existing microtubule via the positively charged and flexible HAUS8 N-terminus, which is essential for microtubule binding23,30. Right, the augmin holocomplex is stabilised and oriented on the microtubule lattice via HAUS6/HAUS7 CH domain binding, as supported by high synergy of the HAUS8 N-terminus and the remaining TII segments in microtubule binding23. Conformational flexibility of the TII clamp (indicated by the black outline) may allow to flexibly adjust the nucleation angle of the branched microtubule.
