Fig. 3: The kinetics of degradation and fusion protein recovery are highly heterogeneous across different technologies.

a Color legend for the genetic targets profiled in this figure. See Supplementary Fig. 9a for more details on the exact constructs profiled in Fig. 3. b Kinetics of degradation after treatment with degrader drugs. After the indicated times, RFluc protein levels were measured via in-cell luciferase signal, while all other protein levels were measured via ICW. Relative protein levels are presented as a fraction of the untreated sample. Errors bars represent the mean and standard error of N = 2 separate wells, representative of N = 2 independent experiments. c Kinetics of recovery after degrader drug washout. After drug treatment (all constructs except SMASh, 24 h; SMASh, 5 days), cells were washed and incubated in a cell culture medium without the drug for the indicated times. RFluc protein levels were measured via in-cell luciferase signal, and error bars represent the standard error of the mean. NanoLuc and PRMT5-CDT protein levels were measured by IB, and data represent the V5 signal normalized to the Vinculin loading control. Errors bars represent the mean of N = 2 technical replicates, representative of N = 2 independent experiments.