Fig. 2: Single-molecule characterization of differently sized α-synuclein aggregates using thioflavin T.

A Histogram of the thioflavin T (ThT) fluorescence intensity distributions for different individual fractions Each histogram is the collected data from three independent gradient centrifugations. B Cumulative frequency distribution for ThT intensity for 20–50% sucrose fractions. Using the Kolmogorov-Smirnov test, the p-values show that the corresponding intensity from each fraction is significantly different from the previous one confirming an increase in size. Kolmogorov-Smirnov test with 20% vs 30% p < 0.001, 30% vs 40% p < 0.001 and 40% vs 50% p < 0.001. (20% N = 3378, 30% N = 8296, 40% N = 11,812, 50% N = 11,816). C–F Representative TIRF images for each fraction including individual zoom-ins. The images were cropped and the contrast was adjusted for clarity (Scale bar = 5 µm). The LUT NanoJ-Orange has been applied to images with a calibration bar. One out of three representative replicates is displayed here. Images were representative across experiments. Each individual colour is representing a distinct sucrose fraction (orange = 20%, green = 30%, blue = 40%, violet = 50%). Source data are provided as a Source Data file. Arb.Units arbitrary units.