Fig. 3: Length distribution of the sucrose fractions from single-molecule super-resolution microscopy using thioflavin X.

A Scatter plot with each point representing one of three biological replicates. Error bars are mean ± STD. Statistical testing using two-tailed paired t-test from N = 3, p = 0.0144 for 20% vs 30%, p = 0.0496 for 30% vs 40%, p = 0.2939 for 40% vs 50%. B Cumulative frequency distribution of α-synuclein aggregates in the correspondent sucrose fractions. One representative out of three independent replicates. C Mean eccentricity for each fraction with each point representing one of three replicates. Error bars are mean ± STD from three independent replicates. D–G Representative super-resolution images from each fraction including their length and eccentricity. The LUT Fire has been applied to all images. Scale bar = 500 nm. Selected representatives out of all three replicates displayed here. Each individual colour is representing a distinct sucrose fraction (orange = 20%, green = 30%, blue = 40%, violet = 50%). Source data are provided as a Source Data file.