Fig. 1: Cytoneme stabilization by Ihog overexpression.
From: Predictive model for cytoneme guidance in Hedgehog signaling based on Ihog- Glypicans interaction
a P compartment cells overexpressing (↑) Ihog in cis (grey) are able to stabilize cytonemes over wild type (WT) A compartment cells (in trans). b P compartment cells overexpressing (↑) Ihog (grey) in cis are able to stabilize cytonemes over the A compartment cells (in trans) where Ihog is downregulated (↓). c P compartment cells overexpressing (↑) Ihog (grey) do not stabilize over the A compartment cells where Ihog is also overexpressed (↑, green) in trans. The schemes at the right depict the cytoneme behavior in the different genetic conditions. d Summary table depicting how cytoneme stabilization depends on Ihog levels in trans. e Boxplot and statistical study was performed using a Wilcoxon rank sum test per pairs over the number of P cytonemes per disc in different experimental conditions. f Orientation of P cytonemes in different conditions shows that P cytonemes are perpendicular to A/P compartment border. Data was quantified from n = 23 discs for section E and from 513 cytonemes of n = 16 discs for section F. In box plots, a box indicates the median (in red and 25 and 75 percentiles, whiskers indicate range of data and crosses indicate outliers. Raw data and p-values (ns:p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001) are provided as a Source data file Scale bars: 15 μm.
