Fig. 3: Cytoneme behavior depends on Ihog and glypicans levels.

a Scheme depicting induced ttv^-/- botv^-/- double mutant clones in a wing disc while expressing also Ihog in the P compartment (green). b Wing disc containing induced ttv^-/- botv^-/- double mutant clones marked by the absence of βgal (b’) while expressing Ihog in the P compartment (b”). Cytonemes stabilization can be observed (white arrow) in A compartment cells except in glypicans loss of function clones induced in the A compartment cells, which do not express Ihog (red arrows). The yellow arrow indicates the presence of stabilized cytonemes when the ttv^-/- botv^-/- double mutant clone is originated in the P compartment, which overexpresses Ihog, indicating that glypicans are required in trans and not in cis for cytoneme stabilization. c Ihog and Dally ectopic clones. Strong cytoneme stabilization is observed when Dally clones are confronted in trans interaction with P compartment cells overexpressing Ihog. c’ Cytonemes are not visualized if Dally clones are in P compartment cells where Ihog is overexpressed (cis interaction). d Ihog and Dlp ectopic clones. Strong cytoneme stabilization is observed when Dlp clones are confronted in trans interaction with P compartment cells overexpressing Ihog. d’ Cytonemes are not visualized when Dlp clones are in the P compartment cells in which Ihog is overexpressed (cis interaction). Experimental sample size: n_Dally = 17 and n_Dlp = 16 discs. Scale bars: 15 μm.