Fig. 4: Ihog and glypicans interaction for cytoneme stabilization in trans is mediated by the Fn1 domain of Ihog.

a Cytonemes protruding from both A and P compartments are strongly stabilized when cells overexpressing glypican Dally (green) are confronted to cells overexpressing wild-type Ihog (red). b Cytoneme bundle stabilization is lost when cells overexpressing Dally (green) are confronted to cells overexpressing the Ihog ΔFn1 mutant form (red). The schemes at the right depict the cytoneme behavior in the different genetic conditions. c Cytonemes protruding from cells overexpressing wild-type Ihog (green) are stabilized when confronted to cells overexpressing the Ihog ΔFn1 mutant form (red). d Boxplot and the statistical study were performed using a Wilcoxon rank sum test per pairs over the number of cytoneme bundles per disc in the different experimental conditions. e) Boxplot and statistical study of the number of P cytonemes per disc in the different experimental conditions shown in Fig. 1c and panel c. Data were quantified from n = 19 discs for section D and from n = 25 discs for section E. In box plots, a box indicates the median (in red) and 25 and 75 percentiles, whiskers indicate range of data and crosses indicate outliers. Raw data and p-values (ns:p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001) are provided as a Source data file. Scale bars: 15 μm.