Fig. 7: Wnt pathway mRNAs are enriched on RPL10A/uL1-containing ribosomes.
From: A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production
a Schematic of 3xFLAG-RPL10A/uL1 and RPL22/eL22-3xFLAG ribosome immunoprecipitation from in vitro differentiated sclerotome and measurements of association with target mRNAs by RT-qPCR. b Genes identified as differentially translated in Rpl10a LOF/LOF embryos showed increased association with RPL10A-uL1-containing ribosomes compared to control transcripts that were unchanged in the ribosome profiling. Data shown are mean +/− SEM. Student’s t test P values: 0.05 (Gapdh), 0.11 (Foxc2), 0.07 (Meox1), 0.08 (Smad4), 0.06 (Tgfbr1), 0.005 (Dhcr24), 0.002 (Fgfr1), 0.007 (Vangl2), 0.12 (Fzd3), 0.003 (Sfrp2), 0.02 (Rac1). *P value < 0.05; **P value < 0.01. Source data are provided as a Source Data file.
