Fig. 9: Rpl10a ^LOF/LOF embryos have reduced noncanonical Wnt/planar cell polarity signaling.

a Lateral views of E12.5 wild-type, Rpl10a ^LOF/+, Vangl2 ^Lp/+, and Rpl10a ^LOF/+; Vangl2 ^Lp/+ embryos and graph of looped tail phenotype frequencies for each genotype. The Rpl10a ^LOF/+; Vangl2 ^Lp/+ embryo shown has the looped tail phenotype (arrow). The increased frequency in Rpl10a ^LOF/+; Vangl2 ^Lp/+ embryos is statistically significant (P = 0.02, Fisher’s exact test). b Schematic of basal foot polarization in the multiciliated cells of the trachea epithelium. Basal feet point in the proximal direction (toward the larynx) in wild-type embryos and are less uniformly oriented when PCP signaling is perturbed. c Transmission electron microscopy of wild-type and Rpl10a ^LOF/LOF E18.5 trachea with basal bodies with visible basal feet demarcated with red arrowheads. Basal feet orientation relative to proximal–distal axis of the trachea, with the oral direction set to 0°, are shown in rose plots with wild-type n = 151 and Rpl10a ^LOF/LOF n = 127. The circular grid lines represent frequencies, and the difference in distribution between wild-type and Rpl10a ^LOF/LOF is statistically significant (Watson’s U² test P value < 0.001). *P value < 0.05; **P value < 0.01. Source data are provided as a Source Data file.