Fig. 2: Interaction of DDs.
From: Metabolic pathway assembly using docking domains from type I cis-AT polyketide synthases
a Purified DDs analyzed using 15.5%-Tris-Tricine-SDS-PAGE. Lane 1: D3 NDD-His-tag (5.1 kDa); lane 2: R5 NDD-His-tag (5.1 kDa); lane 3: Flag-tag-R4 CDD (10.6 kDa); lane M: protein standard marker (P1321, Solarbio); lane 4: Flag-tag-D2 CDD (10.6 kDa); lane 5: Flag-tag-D4 CDD (10.7 kDa); lane 6: D5 NDD-His-tag (6.0 kDa). Experiments were repeated at least three times with similar results. b–d Wavelength shift (nm) generated by the addition of D2 CDD, D4 CDD, or R4 CDD at the concentrations to the complementary D3 NDD, D5 NDD, or R5 NDD, respectively. Source data are provided as a Source Data file.
