Fig. 1: METTL4 mediates solely m6Am methylation of U2 snRNA in Arabidopsis thaliana. | Nature Communications

Fig. 1: METTL4 mediates solely m6Am methylation of U2 snRNA in Arabidopsis thaliana.

From: Structural insights into molecular mechanism for N6-adenosine methylation by MT-A70 family methyltransferase METTL4

Fig. 1

a The diagrammatic sketch of the T-DNA insertion site in the METTL4 gene. b RT-PCR validation of the full-length transcript of the METTL4 gene, the AtACTIN2 transcript acts as a control. c The phenotype of the Arabidopsis plants grown in long-day (LD) condition (22 °C and 16-h-light/8-h-dark). d Statistics of the number of rosette leaves when flower bunds first appeared, which correlates with flowering time. And the number of the calculated plants were shown in the bar column. The APPA mutant was generated from conserved DPPW motif. Wild-type METTL4 and APPA mutant gene rescued mettl4-1 plants are shown as WT-re-mettl4 and Mu-re-mettl4, respectively. ***Significant differences between WT and mutant (Student’s t test: ***P < 0.001). e UPLC-MS/MS chromatograms (left) and quantification of m6Am abundance in total RNA from Arabidopsis thaliana (right). The error bars represent standard deviations. f The quantification of m6Am abundance in U2 snRNA and residual RNAs. g Enzymatic assay of METTL4 using synthetic U2 snRNA fragments and various RNA and DNA oligoes as substrates in vitro. The error bars represent standard deviations. n.d., not detectable; p < 0.001.

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