Fig. 2: C. aquatica transporter mutants modulate tamoxifen toxicity in C. elegans.
From: Bacterial diet modulates tamoxifen-induced death via host fatty acid metabolism
a Schematic of the bacterial genetics screens was performed. b DRCs of tamoxifen toxicity in animals fed C. aquatica mutants identified in the primary screen. Data were represented as mean ± SEM of three independent biological replicates. Statistical analysis of DRCs was conducted by performing two-way ANOVA followed by a Dunnett multi-comparison test using the wild-type E. coli condition as a control on GraphPad Prism (v9). Adjusted p values <0.0001 (E. coli), 0.0415 (tadC−), 0.0169 (acrR−), 0.0456 (exbD−), <0.0001 (hypothetical protein-). *p value <0.05, **p value <0.01, ***p value <0.001. c Schematic of the function of the genes mutated in the strains identified in Fig. 3a, b. Genes encode for factors involved in the type II secretion system (tadC), the TonB-dependent transport (exbD), or the acrA/B multidrug efflux pump (transcriptional repressor acrR). d Quantification of tamoxifen levels accumulating in C. aquatica strains grown on tamoxifen-containing NGM plates. Data were represented as mean ± SEM of three independent biological replicates. Statistical analysis was conducted by performing one-way ANOVA followed by a Dunnett multi-comparison test using the E. coli condition as control on GraphPad Prism (v9). Adjusted p values: 0.0011 (tadC-), 0.7141 (acrR-), 0.5878 (exbD-), and 0.4954 (hypothetical protein-). e Quantification of tamoxifen levels accumulating in wild-type E. coli, C. aquatica, and B. subtilis strains grown on tamoxifen-containing NGM plates. f Quantification of tamoxifen levels in animals fed wild-type E. coli, C. aquatica, and B. subtilis strains in tamoxifen-containing NGM plates.e, f Data were represented as floating bars where the minima bound reaches the minimal value, maxima bound reaches the maximal value, and the center bound is placed at the mean of three independent biological replicates. Statistical analysis of DRCs was conducted by performing a two-way ANOVA followed by a Dunnett multi-comparison test using the C. aquatica wild-type condition as a control on GraphPad Prism (v9). Source data are provided as a Source Data file.
