Fig. 5: Long-chain fatty acid supplementation modulates tamoxifen toxicity.
From: Bacterial diet modulates tamoxifen-induced death via host fatty acid metabolism
a–m Unless otherwise indicated, fatty acids and vitamin E were supplemented to the bacterial culture medium as described previously75, and data were represented as mean ± SEM of three independent biological replicates. Statistical analysis of DRCs was conducted by performing a two-way ANOVA followed by a Dunnett multi-comparison test using the no-supplementation condition as a control, on GraphPad Prism (v9). a–c DRCs of tamoxifen toxicity with and without FA cocktail supplementation on animals fed E. coli (a), C. aquatica (b), or B. subtilis (c). Adjusted p values: 0.9401 (a), 0.0003 (b), and 0.9345 (c). d, e DRCs of tamoxifen toxicity with and without MUFA (VA) supplementation on animals fed E. coli (d) or C. aquatica (e). Adjusted p values: <0.0001 (d) and 0.0708 (e). f Growth curves of B. subtilis supplemented with PUFAs ALA or GLA. For clarity, no error bars are reported in the graph. f, g DRCs of tamoxifen toxicity with and without PUFA (ALA) supplementation on animals fed E. coli (f) or C. aquatica (g). Adjusted p values: 0.9556 (f) and 0.0081 (g). i Growth curves of B. subtilis supplemented with MUFAs VA, PA, or OA. No error bars are reported in the graph. j–l DRCs of tamoxifen toxicity with and without vitamin E supplementation on animals fed E. coli (j), C. aquatica (k), or B. subtilis (l). Adjusted p values: 0.9813 (j), 0.0303 (k), and <0.0001 (l). m DRCs of tamoxifen toxicity with and without the antioxidants vitamin E and N-acetyl-cysteine (NAC). Culture: NAC was supplemented in the bacterial culture medium as for vitamin E. Medium: NAC was supplemented to the NGM. Adjusted p values: <0.0001 (LB + 1% Vit. E), <0.0001 (NGM + NAC, LB + 1% Vit. E), 0.9999 (LB + NAC), 0.9082 (NGM + NAC), and 0.7879 (NGM + NAC, LB + NAC). DRC dose-response curve, FA fatty acid, MUFA mono-unsaturated FA, PUFA poly-unsaturated FA, ALA alpha-linoleic acid, GLA gamma-linoleic acid, DGLA di-homo-gamma-linoleic acid, VA cis-vaccenic acid, PA palmitoleic acid, OA oleic acid. *p value <0.05, **p value <0.01, ***p value <0.001. A larger panel of fatty acid supplements was tested in Supplementary Fig. 7. Source data are provided as a Source Data file.
