Fig. 5: EphA2 signaling impairs IL-23 secretion in BMDCs.

a Correlation of IL-23 kidney levels and cDC infiltration during candidiasis in WT and Epha2^–/– mice. N = 5; combined data of two independent experiments. Pearson correlation coefficient. b CD103^– CD11b⁺ DCs, CD103⁺ CD11b^– DCs, and pDCs in the kidney of wild-type and Epha2^–/– mice after 3 days of infection. Results are median (N = 6) ± SEM of two independent experiments combined. ns; No Significance. Two-tailed Mann–Whitney Test. c IL-23 levels in supernatants of DCs after 24 h stimulation with β-glucan (curdlan). DCs generated from WT and Epha2^–/– mice. N = 6, duplicate. Two-tailed Mann–Whitney Test. Box-and-whisker plots indicating median, 25th/75th percentiles, and the minimum/maximum values. Ctrl, control; BG, β-glucan; Ca, C. albicans. d Number of up- and down-regulated genes in corresponding KEGG pathways. BMDCs from WT and Epha2^–/– mice (N = 3) were stimulated for 6 h with 25 μg/ml curdlan. e DC IL-23 secretion treated with indicated inhibitors and stimulated with curdlan. 24 h post stimulation IL-23 levels in supernatants were measured with ELISA. N = 3; duplicate. Two-tailed Mann–Whitney Test. Box-and-whisker plots indicating median, 25th/75th percentiles, and the minimum/maximum values.