Fig. 8: Recombinant IL-23 treatment reduces ferroptosis and improves disease outcomes during C. albicans infection.
From: IL-23 signaling prevents ferroptosis-driven renal immunopathology during candidiasis
a (Top) IL-23 treatment scheme during disseminated candidiasis. Created with BioRender.com. (Bottom) Survival of wild-type mice infected intravenously with 2.5 × 105 SC5314 C. albicans. N = 7; combined data of two independent experiments. Mice were treated with recombinant murine IL-23 (rmIL-23) or PBS. Mantel–Cox Log-Rank test. b Renal fungal burden after 3 days of infection. Single rmIL-23 treatment at day 2. N = 6 for vehicle-treated mice and N = 7 for rmIL-23-teated mice; combined data of two independent experiments. Mice were treated with recombinant murine IL-23 (rmIL-23) or vehicle (veh; PBS). Two-tailed Mann–Whitney Test. c, d Lipid peroxidation in infected kidneys after 3 days of infection using 4HNE. Single rmIL-23 treatment at day 2 relative to infection. 4HNE shown in green, C. albicans (Ca) in red. Tissue is visualized using DAPI. Scale bar 100 μm. Quantification of mean fluorescence intensity (MFI) per high power field (HPF). N = 6 for vehicle-treated mice and N = 7 for rmIL-23-teated mice; combined data of two independent experiments. Two-tailed Mann–Whitney Test. e Serum NGAL and f TREM1 of infected mice after 3 days of infection. N = 6 for vehicle-treated mice and N = 7 for rmIL-23-teated mice; combined data of two independent experiments. Two-tailed Mann–Whitney Test. g Levels of TNFα, IL-1β, and IL-6 in infected kidneys after 3 days of infection. N = 6 for vehicle-treated mice and N = 7 for rmIL-23-teated mice; combined data of two independent experiments. Two-tailed Mann–Whitney Test. Results are median ± SEM.
