Fig. 2: Dynamical transition of short-range diffusion in cytoplasmic extracts.
From: Cytoplasmic organization promotes protein diffusion in Xenopus extracts
a, Spatial regions of self-organization (color-coded), classified by the ER morphology. The dark sphere near the center of the inner interior is the nuclei. The image was stitched from multiple confocal images. b Representative confocal images of FCS foci, which are marked by the crosshairs. The annotated numbers refer to snapshots taken prior to FCS measurements in c. c Tracing protein diffusion during self-organization by FCS. The plots show the characteristic diffusion times τD and the diffusion mode α obtained from fitting the FCS curves. Black and gray dashed lines correspond to averages from disordered cytoplasmic extracts (in this example, 740 µs, Deff = 11.9 µm2/s) and cytosolic extracts (360 µs, Deff = 24.7 µm2/s), respectively. d Benchmark measurements from cytosolic extracts and buffers. ELB, egg lysis buffer; PBS, Phosphate-buffered saline. Insets show zoom-in of the data. Each condition was measured five times; all data presented by error bars are mean ± SEM. n = 5 measurements of different positions in a sample. e, Biological repeat of c, prepared from a separate batch of eggs. f, g are isolated traces from c and e. Error bars, estimated errors (standard deviation) from curve fitting (each fitting was performed over n = 107 data points). Scale bars, 100 µm. Source data are provided as a Source Data file.
