Fig. 4: Organized cytoplasm optimizes short-range diffusion of protein-sized molecules.
From: Cytoplasmic organization promotes protein diffusion in Xenopus extracts
a Molecular sizes of probes used in this study. b–d Normalized diffusion times of Alexa Fluor 488 dye (AF488), dextran-MW70,000 (Dex-70K), and dextran-MW2,000,000 (Dex-2M) in organized cytoplasmic extracts. These experiments focused on the border regions of the organized cytoplasm such that data collected <10 min could be better compared. Each panel shows data from two droplets of extracts prepared from a single batch of eggs; BSA diffusion was taken as a standard for comparison. Error bars, SEM. n = 4, 6, 4, 5, 6, and 4 measurements of different positions in an extract for data from left to right, respectively. Source data are provided as a Source Data file.
