Fig. 2: Genetic ablation of CD36 in MAMs suppresses liver metastasis.

a The mean fluorescence intensity (MFI) of CD36 in mouse liver-resident cells analyzed by flow cytometry (n = 3). The corresponding Cd36^−/− cells were used as negative control. b CD36 expression in different cell clusters by analyzing the single-cell sequencing data of the liver from Human Protein Atlas Dataset. c HE staining liver sections from Cd36^lko mice and their littermates after intrasplenic injection of LLC cells for 14 days (n = 5). Quantification of tumor area was shown on the right. Scale bar, 400 μm. d, WT and Cd36^−/− mice were treated with clodronate liposomes twice a week after inoculation of tumor cells. On day 14, liver sections were stained with HE and the quantification of tumor area was shown on the right (n = 5). Scale bar, 400 μm. e, Representative livers from Cd36^lysm-cre mice and their littermates after intrasplenic injection of LLC cells (n = 6). The percentage of liver to body weight was shown on the right. f, g HE (f, scale bar, 1000 μm) and Ki67 (g, scale bar, 200 μm) staining in the liver sections from Cd36^lysm-cre mice and their littermates along with the quantification (n = 6). Data are representative of two independent experiments with similar results a, d, e, f. Values for n represent biologically independent samples. Data are mean ± SEM and P values were determined by unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.