Fig. 8: Characterization of inhibitory striatal projection neurons in 5-month-old SNR-derived organoids.

a, Cartoon depicting expression patterns of neuronal marker genes in fetal human telencephalon. b Organoid sections immunostained for GABA, CTIP2, and DARPP32. c Relative distributions of CTIP2- and CTIP2/DARPP32/GABA -expressing cells in organoid section (n = 6 organoids/1–3 sections per organoid produced in three differentiation batches from 2242-5, H9, and GM07492 lines). d Percentages of inhibitory neurons expressing striatal neuron markers (n = 5 [CTIP2] and 3 [DARPP32 and CTIP2] organoids/1–3 sections per organoid, produced in four batches from 2242-5, EP2-15, and H9 lines). e–g Image of GAD67- and FOXP2-expressing neuron (e) that was recorded and filled with biocytin to visualize dendritic spines (f) and morphology (g). h Recordings of voltage deflections obtained in response to Δ10pA somatic current injections from neuron depicted in (e). i Measurements of membrane capacitance, resting membrane potential, latency of 1st action potential, sag amplitude, and action potential adaptation index obtained from recorded cells (n = 34 cells recorded from four organoids obtained in two differentiation batches from 2242-5 and EP2-15 lines). The measurements obtained from neuron depicted in (e) are in red. Data are presented as means ± s.e.m. (d), individual data point and box plots (median ± 25th/75th [box] and min and max values [whiskers]) (c), and individual data points and box plots (median ± 25th/75th [box] and 10th/90th percentiles [whiskers]) (i). Scale bars = 200 and 20 (zoom-in) (b), 50 (e, g), and 5 (f). Source data are provided as a Source Data file.