Fig. 4: SMT2 is required locally for LRFR-induced hypocotyl elongation.
a A simplified representation of sterol biosynthesis pathway in Arabidopsis46. b Normalized CPM (counts per million, normalized to Col-0 average in WL) of SMT2 and SMT3 in the indicated genotypes and conditions (data from RNA-seq). c PIF4-HA binding to the promoter of the indicated genes. PIF4-HA enrichment is quantified by qPCR and presented as IP/Input. Control—C, Peak—P. d–f Hypocotyl elongation of the indicated genotypes. d, f The horizontal bar represents the median; boxes extend from the 25th to the 75th percentile, whiskers extend to show the data range. b, c Each data point indicates biologically (b) or technically (c) independent samples, the horizontal bar represents the median, whiskers extend to show the data range. e Data are means ± SD with a regression line. Different letters (d, f, two-way ANOVA with Tukey’s HSD test) and asterisks (*) (b, c, Student’s T test, one-tailed) (e, two-way ANOVA) indicate a significant difference (P < 0.05) compared to WL (b) or control (c), and between genotypes in given light condition (e). The exact P values are available in the Source Data. Sample size (n) that is given on top (d, f); e for Col-0; n (WL-Mock) = 21, n (WL-10 µM) = 21, n (LB-Mock) = 26, n (LB-10 µM) = 26; n (LRFR-Mock) = 24, n (LRFR-10 µM) = 24, n (LB + LRFR-Mock) = 25, n (LB + LRFR-10 µM) = 25; for smt2-1; n (WL-Mock) = 12, n (WL-10 µM) = 14, n (LB-Mock) = 20, n (LB-10 µM) = 21, n (LRFR-Mock) = 16, n (LRFR-10 µM) = 18, n (LB + LRFR-Mock) = 24, n (LB + LRFR-10 µM) = 18) indicates biologically independent seedlings examined over one experiment. The experiments were repeated two (e) and three (c, d, f) times with similar results. See also Supplementary Fig. 4.
