Fig. 5: Auxin biosynthesis, response, and transport are normal in smt2-1 in LRFR.

a LRFR-induction of auxin biosynthetic genes in the indicated genotypes (data from RNA-seq). Each data point indicates biologically independent samples, the horizontal bar represents the median, whiskers extend to show the data range. b Distributions of Z-scores computed from replicates averages for synthetic auxin picloram up- and downregulated genes in hypocotyls of the indicated genotypes (as listed in ref. 55). c Hypocotyl elongation of indicated genotypes with the indicated doses of picloram. Data are means ± SD with a regression line. d, e Quantification (d) and the representative images (e) of the DII-VENUS signal intensity (normalized to mean value of Col-0 in WL) in hypocotyls of the indicated genotypes either kept at WL or transferred to LRFR for 1 h. White bars equal to 100 µm. b, d The horizontal bar represents the median; boxes extend from the 25th to the 75th percentile, whiskers extend to show the data range. Different letters (d, two-way ANOVA with Tukey’s HSD test) and asterisks (*) (c, two-way ANOVA) indicate a significant difference (P < 0.05) between genotypes in given light condition (c) and compared to WL (d). The exact P values are available in the Source Data. Sample size (n) that is given on top (d); ((c) For Col-0: n (WL) = 17, 22, 21, 20; n (LRFR) = 19, 22, 17, 22. For smt2-1: n (WL) = 12, 15, 19, 17; n (LRFR) = 13, 18, 17, 17 with an order of Mock, 2 µM, 5 µM, 10 µM respectively) indicates biologically independent seedlings examined over one experiment. The experiments were repeated two (c) and three (d) times with similar results. The full gene list of LRFR-picloram transcriptome comparison is given in Supplementary Data 8. See also Supplementary Fig. 5.