Fig. 6: LRFR changes the lipid composition in hypocotyls. | Nature Communications

Fig. 6: LRFR changes the lipid composition in hypocotyls.

From: A combination of plasma membrane sterol biosynthesis and autophagy is required for shade-induced hypocotyl elongation

Fig. 6

a Lipid class abundance at the indicated time points is represented as percentage of total detected lipids in B. rapa hypocotyls. Each data point indicates biologically independent samples, horizontal bar represents the median, whiskers extend to show the data range. GPL glycerophospholipids, GDG glycosyldiacylglycerols, TAG triacylglycerols. b, c Quantification (left) and representative images (right) of (b) LDs using BODIPY™ 493/503 and (c) chloroplasts in Col-0 hypocotyls either kept at WL or transferred to LRFR for 30 h. Data is normalized to WL average. White bars are scale bars of 100 µm. b, c The horizontal bar represents the median; boxes extend from the 25th to the 75th percentile, whiskers extend to show the data range. a–c Asterisks indicate P values (*<0.1, **<0.05, Student’s T test, one-tailed). The exact P values are available in the Source Data. b, c Sample size (n) that is given on top indicates biologically independent seedlings examined over one experiment. The experiments were repeated three times with similar results. The full list of detected lipid species is given in Supplementary Data 9. See also Supplementary Fig. 6.

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