Fig. 1: A developmental time window for organ size adjustment.

a Representative examples of surface reconstruction for volume measurements of the wing pouch domain labeled with nub > GFP at 96 h AED (mid L3 stage), 114 h AED (late L3 stage), and 7 h APF (early pupal stage). Scale bars represent 100 μm. These reconstructions were repeated each time for the n numbers indicated below. b Distribution of the right-left (R-L) pouch volume differences measured for individual pairs of wing discs and expressed as the percentage of the mean pair volume in control dilp8 heterozygous animals (nub > GFP, dilp8^KO/+). **p = 0.0012, Levene’s test. c Distribution of the right-left (R-L) pouch volume differences measured for individual pairs of wing discs and expressed as the percentage of the mean pair volume in null dilp8 mutant animals (nub > GFP, dilp8^KO/KO). ns = not significant, Levene’s test. d FA indexes calculated for each genotype and each timepoint based on the results shown in b and c. ***p = 0.0003 and ns = not significant, Levene’s test. At 96 h AED, n = 29 pairs of discs from independent animals were analyzed for each genotype; at 114 h AED, n = 29 for each genotype; at 7 h APF, n = 37 for dilp8^KO/+, nub > GFP animals and n = 26 for dilp8^KO/KO, nub > GFP animals. AED after egg deposition, APF after pupa formation, L/P transition: larva-to-pupa transition. Source data are provided as a Source Data file.