Fig. 2: A peak of Dilp8 expression at the L/P transition is required for organ size adjustment.

a Measurement of dilp8 mRNA levels by qRT-PCR on whole animals (w¹¹¹⁸ as control strain) at the indicated time points during development. Values are expressed as fold changes relative to the 96 h AED timepoint. Error bars represent SEM. The white prepupa stage (WPP) corresponds to 0 h APF and marks the L/P transition. n = 3 biologically independent samples were analyzed for the 96–116 h AED time points, n = 7 for WPP and n = 4 for the 2–7 h APF stages. b Temporal downregulation of dilp8 using the Shine-GAL4 system, in which the ubi-GAL4^Mag driver is activated upon light exposure. The scheme on the left depicts the different light shifts. Yellow periods mark the developmental times at which animals were switched to white tube light to activate the GAL4/UAS system and downregulate dilp8. Chronic dark and light are the negative and positive controls, respectively. The graph on the right shows the FA indexes measured for adult pairs of wings of the given genotypes after the corresponding shift protocol. This experiment was performed at 29 °C. n values indicate the number of independent wing pairs analyzed; **p = 0.0047, *p = 0.0489 and ns = not significant, Levene’s tests. AED after egg deposition, APF after pupa formation, L/P transition: larva-to-pupa transition, WPP white prepupa, eL3 early L3 stage. Source data are provided as a Source Data file.