Fig. 4: Ecdysone signaling induces dilp8 expression in the epidermis.
From: A Dilp8-dependent time window ensures tissue size adjustment in Drosophila
a Measurement of dilp8 mRNA levels by qRT-PCR on whole animals at the WPP stage upon RNAi-mediated downregulation of the ecdysone receptor gene EcR in the epidermis. Values are expressed as fold changes relative to controls without RNAi. Error bars represent SEM. a: p = 0.0104, b: p = 0.0024, two-tailed t-tests. n = 3 biologically independent samples were analyzed for all genotypes. b Two representative examples of epidermis of animals at the WPP stage showing expression of the dilp8-GFP reporter in control cells and clones of cells expressing a dominant-negative isoform of EcR (EcRDN; cells marked with RFP and shown in red). Scale bars represent 2 μm and DAPI stains the nuclei. This experiment was repeated independently 3 times with similar results. Brightness and contrast adjustments were performed equally on control and experimental conditions and without altering signal localization. c FA indexes of adult wings upon RNAi-mediated downregulation of EcR in the epidermis. n values indicate the number of independent wing pairs analyzed; **p = 0.0033 and *p = 0.0220, two-tailed F-tests. Experiments in a and c were done at 29 °C. WPP white prepupa. Source data are provided as a Source Data file.
