Fig. 2: Phylogenetically conserved TraB family proteins localise to mitochondria and ER. | Nature Communications

Fig. 2: Phylogenetically conserved TraB family proteins localise to mitochondria and ER.

From: TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy

Fig. 2

a Phylogenetic tree of TraB family homologues showing that Arabidopsis TRB1 and TRB2 originate from the same clade, whereas TRB3 belongs to a separate clade that is more evolutionarily similar to their mammalian homologues. b In Arabidopsis, GFP-TRB1 localizes to the outer membrane of mitochondria that is labelled by the mitochondrial matrix marker, Mito-mCherry. The inset are additional images taken by high-resolution microscopy showing clear mitochondrial membrane localisation of TRB1. c, d GFP-TRB2 localizes to the outer mitochondrial membrane as well as the ER network (mTAGBFP2-HDEL labelled). e RFP-TRB3 mostly localizes to the cytoplasm, its signal is clearly distinct from the mitochondria maker. f In citrus, only one isoform of TraB family proteins containing TMD exists, which also localizes to the outer mitochondrial membrane in N. benthamiana leaf epidermal cells. Microscopy studies were repeated two times for c and at least three times for b, d–f (scale bar = 10 μm).

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