Fig. 4: GDYO directly interacted with ITGB2 and MRC2 in DNMT3A mutant cells.

a Heatmap of GDYO-interacted proteins detected by LC-MS/MS, n = 2. b Interaction network of key proteins. c Gene expression of proteins in key pathways between DNMT3A^mut and DNMT3A^wt human AML cell lines from CCLE database. DNMT3A^mut, n = 4; DNMT3A^wt, n = 30. d Antibody-guided immunoblot verified expression of selected proteins. e, g Side view of molecular dynamic (MD) simulation showing the adsorption of the ITGB2 (e) and MRC2 (g) proteins onto GDYO nanosheets. f, h The stable absorption residues of the ITGB2 (f) and MRC2 (h) proteins onto GDYO nanosheets. The numbers in bracket represent the hydrophobic parameters of corresponding residues. i Sample preparation diagram of to prepare GDYO^B/GDYO^M and to detect the interacted receptors. j Antibody-guided immunoblot for binding ability between ITGB2/MRC2 and GDYO/GDYO^B/GDYO^M. k Sample preparation diagram to profile proteins absorbed on GDYO^B/GDYO^M. l Protein content quantification of proteins absorbed on GDYO^B/GDYO^M. n = 3 biologically independent experiments. m Venn diagram of protein species of proteins absorbed on GDYO^B/GDYO^M. n Top 15 abundant proteins absorbed on GDYO^B/GDYO^M. n = 3 biologically independent experiments. The data were shown as the mean ± SD. Statistical significance was tested with a two-tailed, unpaired Student’s t test. Source data are provided as a Source Data file.