Fig. 2: Shifts in the abundances of bacterial taxa in AiMS reactions with grain from the IS3620C or BTx623 parental lines.

IS3620C and BTx623 are the parental lines of sorghum used to generate the RIL population used in our study. The left-hand portion of the figure illustrates microbial taxa from 12 different human subjects that show significant differences in abundances between fermentation of grain from the IS3620C or BTx623 parents. The microbial taxa are organized based on a neighbor-joining tree representing their phylogenetic relationships. These phylogenetic relationships were inferred from representative sequences of species from each bacterial genus using MUltiple Sequence Comparison by Log- Expectation (MUSCLE). Bacterial genera from the same family are shaded in the same colors. On the right-hand portion of the figure, abundance differences of each taxon in AiMS fermentations from each subject are  depicted by heat-mapping of the mean log2-transformed-fold difference from fermentation of grain from the IS3620C parent relative to BTx623 parent. All taxa illustrated show significant microbiome-wide effects across the human subjects (unadjusted p < 0.05; two-sided rANOVA) and statistically significant differences between IS3620C and BTx623 sorghum for each taxon were determined by subject microbiome (Two-sided Wilcoxon test; p < 0.05) and denoted by an asterisk. Source data are provided as a Source Data file.