Fig. 4: Tracking of the delivery of self-assembled TNF-α siRNA to immune cells in inflamed mucosa.
From: In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis
a Kinetics of TNF-α siRNA in the mouse colon, spleen and kidney following tail vein injection of the 5 mg/kg CMV-siRTNF-α circuit (n = 3 in each group). The total TNF-α siRNA levels in the colon, spleen and kidney were calculated based on the total amount of RNA isolated from one gram of each tissue and the TNF-α siRNA content in one μg of total RNA. b In situ detection of TNF-α siRNA in colon sections of DSS mice at 0, 6, 12, 24 or 48 h after injection with the CMV-siRTNF-α circuit. Positive in situ hybridisation signals are shown in green, and DAPI-stained nuclei are shown in blue. Scale bar: 50 μm. c Kinetics of TNF-α siRNA in monocytes and macrophages derived from peripheral blood, spleen and colonic lamina propria of DSS mice following tail vein injection of the 5 mg/kg CMV-siRTNF-α circuit (n = 3 in each group). d Kinetics of TNF-α siRNA in CD4+ T cells derived from peripheral blood, spleen and colonic lamina propria of DSS mice following tail vein injection of the 5 mg/kg CMV-siRTNF-α circuit (n = 3 in each group).
